A comprehensive study of biocompatibility of detonation nanodiamonds

Journal article

The article describes a complex study of detonation nanodiamonds (DND) aqueous dispersions. In this research, DND sample was characterised by means of IR, NMR spectroscopy, TEM, thermogravimetric analysis, size distribution, and ζ-potentials. It was shown that DND sample includes several surface groups, mainly hydroxylic, carboxylic, and carbonyl ones. Dynamic light scattering results revealed that in the concentration range C = 0.002–0.3 wt%, DND nanoparticles size is equal to 55 ± 5 nm. It was demonstrated that DND possessed weak antiradical activity, had an inhibitory effect on F1F0-ATPase activity, almost did not affect platelet aggregation, formed a stronger complex with human serum albumin (HSA) in subdomain IB (digitoxin, Kb = 20.0 ± 2.4 l·g−1) and a less strong complex in subdomain IIA (warfarin, Kb = 3.7 ± 0.1 l·g−1), inhibited the esterase activity of HSA, DND dispersions (C = 0.0012–0.15 wt%) revealed genotoxic effect towards PBMCs, did not affect cellular proliferation in the experiment with HEK293 cell line, did not reveal cytotoxic effect up to 0.01 wt%. Using DFT and MD approaches allowed us to perform a simulation of interaction between DND nanoparticle and water molecules.

Authors: Berdichevskiy, GM; Vasina, LV; Ageev, SV; Meshcheriakov, AA; Galkin, MA

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: Elsevier
• Journal: Journal of Molecular Liquids
• Volume: 332
• Article number: 115763
• Publication date: 2021-02-26
• Acceptance date: 2021-02-22
• DOI: 10.1016/j.molliq.2021.115763
• EISSN: 1873-3166
• ISSN: 0167-7322
• Language: English
• Keywords: HSA; DPPH; FFR; platelets; genotoxicity; detonation nanodiamonds; molecular dynamics