Conference item

Identification of viable and non-viable haemophilus influenzae using qPCR

Abstract:: Conventional real-time PCR for the detection of bacteria is unable to distinguish between viable and non-viable cells within a sample. Propidium monoazide (PMA) is a photo-reactive dye that is cell membrane impermeable which interacts with non-viable cells ceasing amplification due to DNA modification. We investigated if PMA could distinguish between viable and non-viable Haemophilus influenzae.

Publication status:: Published

Peer review status:: Peer reviewed

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APA Style

Thulborn, S., & Bafadhel, M. (2016). Identification of viable and non-viable haemophilus influenzae using qPCR. European Respiratory Journal, 48(suppl 60), PA2622.

MLA Style

Thulborn, S., and M. Bafadhel. “Identification of Viable and Non-Viable Haemophilus Influenzae Using QPCR.” European Respiratory Journal, vol. 48, no. suppl 60, European Respiratory Society, 2016, p. PA2622.

Chicago Style

Thulborn, S, and M Bafadhel. 2016. “Identification of Viable and Non-Viable Haemophilus Influenzae Using QPCR.” In European Respiratory Journal, 48:PA2622. European Respiratory Society.
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Files:: Identification of viable and non-viable haemophilus influenzae...

(Preview, Accepted manuscript, pdf, 408.9KB, Terms of use)

Publisher copy:: 10.1183/13993003.congress-2016.PA2622

Authors

+ Thulborn, S More by this author

Institution:: University of Oxford
Division:: Medical Sciences Division
Department:: NDM
Sub department:: NDM Experimental Medicine
Role:: Author

+ Bafadhel, M More by this author

Institution:: University of Oxford
Division:: MSD
Department:: NDM
Sub department:: Target Discovery Institute
Role:: Author
ORCID:: 0000-0002-9993-2478

Publisher:: European Respiratory Society
Host title:: European Respiratory Journal
Journal:: European Respiratory Society International Congress 2016 More from this journal
Volume:: 48
Issue:: suppl 60
Pages:: PA2622
Publication date:: 2016-11-08
Acceptance date:: 2016-08-01
DOI:: 10.1183/13993003.congress-2016.PA2622
EISSN:: 1399-3003
ISSN:: 0903-1936

Keywords:: COPD - exacerbations

infections

bacteria
Pubs id:: pubs:921683
UUID:: uuid:8a5bcc98-4486-44de-a218-478767cf417e
Local pid:: pubs:921683
Source identifiers:: 921683
Deposit date:: 2018-10-04

Terms of use

Copyright holder:: Thulborn and Bafadhel
Copyright date:: 2016
Notes:: Copyright ©the authors 2016. This is the accepted manuscript version of the article. The final version is available online from the European Respiratory Society at: https://doi.org/10.1183/13993003.congress-2016.PA2622

Licence:: Terms and Conditions of Use for Oxford University Research Archive

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