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Conference item

Identification of viable and non-viable haemophilus influenzae using qPCR

Abstract:
Conventional real-time PCR for the detection of bacteria is unable to distinguish between viable and non-viable cells within a sample. Propidium monoazide (PMA) is a photo-reactive dye that is cell membrane impermeable which interacts with non-viable cells ceasing amplification due to DNA modification. We investigated if PMA could distinguish between viable and non-viable Haemophilus influenzae.
Publication status:
Published
Peer review status:
Peer reviewed

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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
NDM
Sub department:
NDM Experimental Medicine
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Target Discovery Institute
Role:
Author
ORCID:
0000-0002-9993-2478
Publisher:
European Respiratory Society
Host title:
European Respiratory Journal
Journal:
European Respiratory Society International Congress 2016 More from this journal
Volume:
48
Issue:
suppl 60
Pages:
PA2622
Publication date:
2016-11-08
Acceptance date:
2016-08-01
DOI:
EISSN:
1399-3003
ISSN:
0903-1936
Keywords:
Pubs id:
pubs:921683
UUID:
uuid:8a5bcc98-4486-44de-a218-478767cf417e
Local pid:
pubs:921683
Source identifiers:
921683
Deposit date:
2018-10-04

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