Book section : Chapter
Combining whole-cell patch-clamp recordings with single-cell RNA sequencing
- Abstract:
- To understand how the brain functions we need to understand the properties of its constituent cells. Whole-cell patch-clamp recordings of neurons have enabled studies of their intrinsic electrical properties as well as their synaptic connectivity within neural circuits. Recent technological advances have now made it possible to combine this with a sampling of their transcriptional profile. Here we provide a detailed description how to combine whole-cell patch-clamp recordings of neurons in brain slices followed by extraction of their cytoplasm suitable for single-cell RNA sequencing and analysis.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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Access Document
- Files:
-
-
(Preview, Accepted manuscript, pdf, 1.1MB, Terms of use)
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- Publisher copy:
- 10.1007/978-1-0716-0818-0_9
- Publisher:
- Springer US
- Host title:
- Patch Clamp Electrophysiology
- Pages:
- 179-189
- Series:
- Methods in Molecular Biology
- Series number:
- 2188
- Place of publication:
- United States
- Publication date:
- 2020-10-30
- DOI:
- EISSN:
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1940-6029
- ISSN:
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1064-3745
- Pmid:
-
33119852
- EISBN:
- 978-1-0716-0818-0
- ISBN:
- 9781071608173
- Language:
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English
- Keywords:
- Subtype:
-
Chapter
- Pubs id:
-
1141501
- Local pid:
-
pubs:1141501
- Deposit date:
-
2021-07-17
Terms of use
- Copyright holder:
- Springer Science+Business Media, LLC
- Copyright date:
- 2021
- Rights statement:
- © Springer Science+Business Media, LLC, part of Springer Nature 2021
- Notes:
- This is the accepted manuscript version of the chapter. The final version is available online from Springer US at https://dx.doi.org/10.1007/978-1-0716-0818-0_9
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