Development of an image-based system for measurement of membrane potential, intracellular Ca(2+) and contraction in arteriolar smooth muscle cells.

Journal article

OBJECTIVE: Changes in smooth muscle cell (SMC) membrane potential (Em) are critical to vasomotor responses. As a fluorescent indicator approach would lessen limitations of glass electrodes in contracting preparations, we aimed to develop a Forster (or fluorescence) resonance energy transfer (FRET)-based measurement for Em. METHODS: The FRET pair used in this study (donor CC2-DMPE [excitation 405 nm] and acceptor DisBAC(4) (3)) provide rapid measurements at a sensitivity not achievable with ma...

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Authors: Ella, SR; Yang, Y; Clifford, P; Gulia, J; Dora, K

• Publication status: Published
• Journal: Microcirculation (New York, N.Y. : 1994)
• Volume: 17
• Issue: 8
• Pages: 629-640
• Publication date: 2010-11-01
• DOI: 10.1111/j.1549-8719.2010.00059.x
• EISSN: 1549-8719
• ISSN: 1073-9688
• Language: English
• Keywords: Humans; Barbiturates; Muscle Contraction; Ethanolamines; Fluorescence Resonance Energy Transfer; Animals; Myocytes, Smooth Muscle; Male; HEK293 Cells; Arterioles; Calcium; Patch-Clamp Techniques; Fluorescent Dyes; Rats, Sprague-Dawley; Coumarins; Membrane Potentials; Image Processing, Computer-Assisted; In Vitro Techniques; Rats; Isoxazoles