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Development of an image-based system for measurement of membrane potential, intracellular Ca(2+) and contraction in arteriolar smooth muscle cells.

Abstract:

OBJECTIVE: Changes in smooth muscle cell (SMC) membrane potential (Em) are critical to vasomotor responses. As a fluorescent indicator approach would lessen limitations of glass electrodes in contracting preparations, we aimed to develop a Forster (or fluorescence) resonance energy transfer (FRET)-based measurement for Em. METHODS: The FRET pair used in this study (donor CC2-DMPE [excitation 405 nm] and acceptor DisBAC(4) (3)) provide rapid measurements at a sensitivity not achievable with ma...

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Publication status:
Published

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Institution:
University of Oxford
Division:
MSD
Department:
Pharmacology
Role:
Author
Journal:
Microcirculation (New York, N.Y. : 1994) More from this journal
Volume:
17
Issue:
8
Pages:
629-640
Publication date:
2010-11-01
DOI:
EISSN:
1549-8719
ISSN:
1073-9688

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