Journal article
A protocol for dual calcium-voltage optical mapping in murine sinoatrial preparation with optogenetic pacing
- Abstract:
- Among the animal models for studying the molecular basis of atrial and sinoatrial node (SAN) biology and disease, the mouse is a widely used species due to its feasibility for genetic modifications in genes encoding ion channels or calcium handling and signaling proteins in the heart. It is therefore highly valuable to develop robust methodologies for studying SAN and atrial electrophysiological function in this species. Here, we describe a protocol for performing dual calcium-voltage optical mapping on mouse sinoatrial preparation (SAP), in combination with an optogenetic approach, for studying SAP membrane potential, intracellular Ca2+ transients, and pacemaker activity. The protocol includes the details for preparing the intact SAP, robust tissue dual-dye loading, light-programmed pacing, and high-resolution optical mapping. Our protocol provides an example of use of the combination of optogenetic and optical mapping techniques for investigating SAP membrane potential and intracellular Ca2+ transients and pacemaker activity with high temporal and spatial resolution in specific cardiac tissues. Thus, our protocol provides a useful tool for studying SAP physiology and pathophysiology in mice.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
Actions
Access Document
- Files:
-
-
(Preview, Version of record, pdf, 2.0MB, Terms of use)
-
- Publisher copy:
- 10.3389/fphys.2019.00954
Authors
- Publisher:
- Frontiers Media
- Journal:
- Frontiers in physiology More from this journal
- Volume:
- 10
- Article number:
- 954
- Publication date:
- 2019-08-06
- Acceptance date:
- 2019-07-09
- DOI:
- EISSN:
-
1664-042X
- Pmid:
-
31456689
- Language:
-
English
- Keywords:
- Pubs id:
-
pubs:1047044
- UUID:
-
uuid:8a20f854-d97b-486b-9053-276fad6700f7
- Local pid:
-
pubs:1047044
- Source identifiers:
-
1047044
- Deposit date:
-
2019-10-16
Terms of use
- Copyright holder:
- Dong et al
- Copyright date:
- 2019
- Notes:
- Copyright © 2019 Dong, Mu-u-min, Reith, O’Shea, He, Duan, Kou, Grassam-Rowe, Tan, Pavlovic, Ou and Lei. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
- Licence:
- CC Attribution (CC BY)
If you are the owner of this record, you can report an update to it here: Report update to this record