Journal article
Improved workflows for high throughput library preparation using the transposome-based Nextera system.
- Abstract:
-
BACKGROUND: The Nextera protocol, which utilises a transposome based approach to create libraries for Illumina sequencing, requires pure DNA template, an accurate assessment of input concentration and a column clean-up that limits its applicability for high-throughput sample preparation. We addressed the identified limitations to develop a robust workflow that supports both rapid and high-throughput projects also reducing reagent costs. RESULTS: We show that an initial bead-based normalisatio...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
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Funding
Bibliographic Details
- Publisher:
- BioMed Central Publisher's website
- Journal:
- BMC biotechnology Journal website
- Volume:
- 13
- Issue:
- 1
- Pages:
- 104
- Publication date:
- 2013-01-01
- DOI:
- EISSN:
-
1472-6750
- ISSN:
-
1472-6750
Item Description
- Language:
- English
- Keywords:
- UUID:
-
uuid:88f1351b-4617-4a77-978c-4cffa2529933
- Local pid:
- pubs:440271
- Source identifiers:
-
440271
- Deposit date:
- 2013-12-12
Terms of use
- Copyright holder:
- Lamble et al
- Copyright date:
- 2013
- Notes:
- © 2013 Lamble et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
- Licence:
- CC Attribution (CC BY)
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