Targeted transfection by femtosecond laser.

Journal article

The challenge for successful delivery of foreign DNA into cells in vitro, a key technique in cell and molecular biology with important biomedical implications, is to improve transfection efficiency while leaving the cell's architecture intact. Here we show that a variety of mammalian cells can be directly transfected with DNA without perturbing their structure by first creating a tiny, localized perforation in the membrane using ultrashort (femtosecond), high-intensity, near-infrared laser pu...

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Authors: Tirlapur, U; König, K

• Publication status: Published
• Journal: Nature
• Volume: 418
• Issue: 6895
• Pages: 290-291
• Publication date: 2002-07-01
• DOI: 10.1038/418290a
• EISSN: 1476-4687
• ISSN: 0028-0836
• Language: English
• Keywords: Animals; Transgenes; Infrared Rays; Lasers; Vaccines, DNA; Cell Membrane; Luminescent Proteins; Macropodidae; Transfection; Gene Expression; Genetic Therapy; Cell Line; Culture Media; Fluorescence; Cell Survival; Green Fluorescent Proteins; DNA; Genes, Reporter; Cricetinae; CHO Cells