Mediation of Af4 protein function in the cerebellum by Siah proteins.

Journal article

We have established that the gene AF4, which had long been recognized as disrupted in childhood leukemia, also plays a role in the CNS. Af4 is mutated in the robotic mouse that is characterized by ataxia and Purkinje cell loss. To determine the molecular basis of this mutation, we carried out a yeast two-hybrid screen and show that Af4 binds the E3 ubiquitin ligases Drosophila seven in absentia (sina) homologues (Siah)-1a and Siah-2 in the brain. Siah-1a and Af4 are expressed in Purkinje cell...

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Authors: Oliver, P; Bitoun, E; Clark, J; Jones, E; Davies, K

• Journal: Proc Natl Acad Sci U S A
• Volume: 101
• Issue: 41
• Pages: 14901-14906
• Publication date: 2004-10-12
• DOI: 10.1073/pnas.0406196101
• EISSN: 1091-6490
• ISSN: 0027-8424
• Language: English
• Keywords: Humans; Ubiquitin-Protein Ligases; Amino Acid Sequence; Sequence Homology, Amino Acid; Mutation; Drosophila Proteins; Cell Line; Drosophila; Mice; Molecular Sequence Data; DNA-Binding Proteins; Nuclear Proteins; Sequence Alignment; Transfection; Animals; Cell Line, Tumor