Production of a functional anti-scorpion hemocyanin scFv in Escherichia coli.

Journal article

A58 is a murine monoclonal antibody raised against the internal dimeric subunit A alpha 1 of the hemocyanin of the scorpion Androctonus australis. When A58 is tested against a range of related hemocyanins, it exhibits cross-reactivity with a limited number of scorpion species. All of them belong to a single Scorpion Family: the Buthidae. We have utilized a bacterial expression-export system in which the variable regions of A58 are expressed as a single-chain variable fragment (scFv). Polymera...

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Authors: Billiald, P; Motta, G; Vaux, D

• Publication status: Published
• Journal: Archives of biochemistry and biophysics
• Volume: 317
• Issue: 2
• Pages: 429-438
• Publication date: 1995-03-01
• DOI: 10.1006/abbi.1995.1185
• EISSN: 1096-0384
• ISSN: 0003-9861
• Language: English
• Keywords: Polymerase Chain Reaction; Amino Acid Sequence; Antibodies, Monoclonal; Cloning, Molecular; Escherichia coli; Sequence Analysis, DNA; Scorpions; Antibody Specificity; Macromolecular Substances; Recombinant Proteins; Mice; Molecular Sequence Data; Gene Expression; Restriction Mapping; Hemocyanin; Immunoglobulin Variable Region; Genetic Vectors; Base Sequence; Animals