- Abstract:
-
We describe a protocol for forming an artificial lipid bilayer by contacting nanoliter aqueous droplets in an oil solution in the presence of phospholipids. A lipid monolayer forms at each oil-water interface, and when two such monolayers touch, a bilayer is created. Droplet interface bilayers (DIBs) are a simple way to generate stable bilayers suitable for single-channel electrophysiology and optical imaging from a wide variety of preparations, ranging from purified proteins to reconstituted...
Expand abstract - Publication status:
- Published
- Publisher copy:
- 10.1038/nprot.2013.061
-
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- Journal:
- Nature protocols
- Volume:
- 8
- Issue:
- 6
- Pages:
- 1048-1057
- Publication date:
- 2013-06-05
- DOI:
- EISSN:
-
1750-2799
- ISSN:
-
1754-2189
- URN:
-
uuid:86218d23-3c29-4a52-b81c-97e9886a673d
- Source identifiers:
-
399351
- Local pid:
- pubs:399351
- Language:
- English
- Keywords:
- Copyright date:
- 2013
Journal article
Constructing droplet interface bilayers from the contact of aqueous droplets in oil.
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