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Constructing droplet interface bilayers from the contact of aqueous droplets in oil.

Abstract:

We describe a protocol for forming an artificial lipid bilayer by contacting nanoliter aqueous droplets in an oil solution in the presence of phospholipids. A lipid monolayer forms at each oil-water interface, and when two such monolayers touch, a bilayer is created. Droplet interface bilayers (DIBs) are a simple way to generate stable bilayers suitable for single-channel electrophysiology and optical imaging from a wide variety of preparations, ranging from purified proteins to reconstituted...

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Publication status:
Published

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Publisher copy:
10.1038/nprot.2013.061

Authors


Leptihn, S More by this author
Castell, OK More by this author
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Journal:
Nature protocols
Volume:
8
Issue:
6
Pages:
1048-1057
Publication date:
2013-06-05
DOI:
EISSN:
1750-2799
ISSN:
1754-2189
URN:
uuid:86218d23-3c29-4a52-b81c-97e9886a673d
Source identifiers:
399351
Local pid:
pubs:399351

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