Proton-selective conductance and gating of the lysosomal cation channel TMEM175

Journal article

The lysosomal cation channel TMEM175 plays a key role in luminal pH homeostasis and lysosome function, with aberrant activity linked to Parkinson’s disease. Although initially described as a K+-selective channel, TMEM175 exhibits substantial H+ permeability. Here, we dissect complex changes affecting human TMEM175 conductance and ionic properties of TMEM175-mediated current in response to pH shifts on the luminal side of the protein. A drop in pH from 7.4 to 4.7 on the side equivalent to the lysosomal lumen triggers a sustained increase in TMEM175-mediated inward and outward currents, which is accompanied by a transient shift in the reversal potential (Erev) toward the theoretical equilibrium voltage for H+, yet remaining ~100 mV below the expected value even in the absence of K+. This discrepancy, along with low sensitivity of Erev to the concentration gradient for K+, supports a model in which TMEM175-mediated H+ flux rapidly collapses the lysosomal pH-gradient. Molecular dynamics simulations identify H57 as a key residue on the luminal side of the open channel, which forms intra- and intersubunit salt bridges with D279 and E282. Supporting the functional importance of these interactions, the TMEM175 mutant H57Y displayed reduced H+- and K+-conductance and a reduced H+/K+ selectivity in whole-cell and lysosomal electrophysiological analyses. Our findings contribute to a better understanding of TMEM175’s complex electrophysiological properties, thereby expanding the possibilities of understanding the channel’s function in lysosomal physiology and pathophysiology.

Authors: Schulze, T; Sprave, T; Groebe, C; Hendrik Krumbach, J; Behringer, M

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: National Academy of Sciences
• Journal: Proceedings of the National Academy of Sciences
• Volume: 123
• Issue: 3
• Article number: e2503909123
• Publication date: 2026-01-14
• Acceptance date: 2025-11-18
• DOI: 10.1073/pnas.2503909123
• EISSN: 1091-6490
• ISSN: 0027-8424
• Language: English
• Keywords: patch-clamp; SSME; MD simulations; TMEM175; proton channel