H₂-driven cofactor regeneration with NAD(P)⁺-reducing hydrogenases.

Journal article

A large number of industrially relevant enzymes depend upon nicotinamide cofactors, which are too expensive to be added in stoichiometric amounts. Existing NAD(P)H-recycling systems suffer from low activity, or the generation of side products. H₂-driven cofactor regeneration has the advantage of 100% atom efficiency and the use of H₂ as a cheap reducing agent, in a world where sustainable energy carriers are increasingly attractive. The state of development of H₂-driven cofactor-recycling systems and examples of their integration with enzyme reactions are summarized in this article. The O₂-tolerant NAD⁺-reducing hydrogenase from Ralstonia eutropha is a particularly attractive candidate for this approach, and we therefore discuss its catalytic properties that are relevant for technical applications.

Authors: Lauterbach, L; Lenz, O; Vincent, K

• Publication status: Published
• Journal: FEBS journal
• Volume: 280
• Issue: 13
• Pages: 3058-3068
• Publication date: 2013-07-01
• DOI: 10.1111/febs.12245
• EISSN: 1742-4658
• ISSN: 1742-464X
• Language: English
• Keywords: Enzymes, Immobilized; NADP; Cupriavidus necator; NAD; Desulfovibrio; Bioreactors; Oxidation-Reduction; Bacterial Proteins; Enzyme Stability; Hydrogen; Biocatalysis; Oxidoreductases; Archaeal Proteins; Reducing Agents