A new apparatus to induce lysis of planktonic microbial cells by shock compression, cavitation and spray

Journal article

Experiments were conducted on an aqueous growth medium containing cultures of Escherichia coli (E. coli) XL1-Blue, to investigate, in a single experiment, the effect of two types of dynamic mechanical loading on cellular integrity. A bespoke shock tube was used to subject separate portions of a planktonic bacterial culture to two different loading sequences: (i) shock compression followed by cavitation, and (ii) shock compression followed by spray. The apparatus allows the generation of an adjustable loading shock wave of magnitude up to 300 MPa in a sterile laboratory environment. Cultures of E. coli were tested with this apparatus and the spread-plate technique was used to measure the survivability after mechanical loading. The loading sequence (ii) gave higher mortality than (i), suggesting that the bacteria are more vulnerable to shear deformation and cavitation than to hydrostatic compression. We present results of preliminary experiments and suggestions for further experimental work; we discuss the potential applications of this technique to sterilise large volumes of fluid samples.

Authors: Schiffer, A; Gardner, M; Tagarielli, V; Lynn, R

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: Royal Society
• Journal: Royal Society Open Science
• Volume: 4
• Pages: 160939
• Publication date: 2017-03-01
• Acceptance date: 2017-02-14
• DOI: 10.1098/rsos.160939
• ISSN: 2054-5703
• Keywords: cavitation; shock wave; Escherichia coli; cell lysis