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Comparative structural, kinetic and inhibitor studies of Trypanosoma brucei trypanothione reductase with T. cruzi

Abstract:

As part of a drug discovery programme to discover new treatments for human African trypanosomiasis, recombinant trypanothione reductase from Trypanosoma brucei has been expressed, purified and characterized. The crystal structure was solved by molecular replacement to a resolution of 2.3 Å and found to be nearly identical to the T. cruzi enzyme (root mean square deviation 0.6 Å over 482 Cα atoms). Kinetically, the Km for trypanothione disulphide for the T. brucei enzyme was 4.4-fold lower tha...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's Version

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Publisher copy:
10.1016/j.molbiopara.2009.09.002

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Department:
Oxford, MSD, Pathology Dunn School
Role:
Author
Publisher:
Elsevier Publisher's website
Journal:
Molecular and Biochemical Parasitology Journal website
Volume:
169
Issue:
1
Pages:
12-19
Publication date:
2010-01-05
DOI:
EISSN:
1872-9428
ISSN:
0166-6851
Pubs id:
pubs:659853
URN:
uri:8208d33e-c850-4484-8ef7-3a7487eee600
UUID:
uuid:8208d33e-c850-4484-8ef7-3a7487eee600
Local pid:
pubs:659853
Language:
English
Keywords:

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