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Investigations into the post-translational modification and mechanism of isopenicillin N:acyl-CoA acyltransferase using electrospray mass spectrometry.

Abstract:

Electrospray mass spectrometry (e.s.m.s.) was used to confirm the position of the post-translational cleavage of the isopenicillin N:acyl-CoA acyltransferase preprotein to give the alpha- and beta-subunits. The e.s.m.s. studies suggested partial modification of the alpha-subunit in vivo by exogenously added substituted acetic acids. E.s.m.s. has also allowed the observation in vitro of the transfer of the acyl group from several acyl-CoAs to the beta-subunit. N.m.r. data for the CoA species h...

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Publication status:
Published

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Authors


Baldwin, JE More by this author
Robinson, CV More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Organic Chemistry
Journal:
The Biochemical journal
Volume:
294 ( Pt 2)
Pages:
357-363
Publication date:
1993-09-05
EISSN:
1470-8728
ISSN:
0264-6021
URN:
uuid:80d65546-8af6-41b6-b2fb-9c0297abfb0e
Source identifiers:
35853
Local pid:
pubs:35853

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