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Journal article

Identification and characterization of a heterotrimeric archaeal DNA polymerase holoenzyme

Abstract:

Since their initial characterization over 30 years ago, it has been believed that the archaeal B-family DNA polymerases are single-subunit enzymes. This contrasts with the multi-subunit B-family replicative polymerases of eukaryotes. Here we reveal that the highly studied PolB1 from Sulfolobus solfataricus exists as a heterotrimeric complex in cell extracts. Two small subunits, PBP1 and PBP2, associate with distinct surfaces of the larger catalytic subunit and influence the enzymatic properti...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1038/ncomms15075

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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Pathology Dunn School
Role:
Author
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Pathology Dunn School
Role:
Author
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Publisher:
Springer Nature Publisher's website
Journal:
Nature Communications Journal website
Volume:
8
Pages:
15075
Publication date:
2017-05-02
Acceptance date:
2017-02-27
DOI:
EISSN:
2041-1723
ISSN:
2041-1723
Pubs id:
pubs:693412
URN:
uri:80378446-e305-47f0-8601-79727a0c38da
UUID:
uuid:80378446-e305-47f0-8601-79727a0c38da
Local pid:
pubs:693412

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