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Changing metabolism in differentiating cardiac progenitor cells - can stem cells become metabolically flexible cardiomyocytes?

Abstract:
The heart is a metabolic omnivore and the adult heart selects the substrate best suited for each circumstance, with fatty acid oxidation preferred in order to fulfill the high energy demand of the contracting myocardium. The fetal heart exists in an hypoxic environment and obtains the bulk of its energy via glycolysis. After birth, the “fetal switch” to oxidative metabolism of glucose and fatty acids has been linked to the loss of the regenerative phenotype. Various stem cell types have been used in differentiation studies, but most are cultured in high glucose media. This does not change in the majority of cardiac differentiation protocols. Despite the fact that metabolic state affects marker expression and cellular function and activity, the substrate composition is currently being overlooked. In this review we discuss changes in cardiac metabolism during development, the various protocols used to differentiate progenitor cells to cardiomyocytes, what is known about stem cell metabolism and how consideration of metabolism can contribute toward maturation of stem cell-derived cardiomyocytes.
Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.3389/fcvm.2018.00119

Authors


More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Physiology Anatomy and Genetics
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Physiology Anatomy & Genetics
Oxford college:
Pembroke College
Role:
Author
ORCID:
0000-0003-0102-5261


Publisher:
Frontiers Media
Journal:
Frontiers in Cardiovascular Medicine More from this journal
Volume:
5
Pages:
119
Publication date:
2018-09-19
Acceptance date:
2018-08-10
DOI:
EISSN:
2297-055X


Keywords:
Pubs id:
pubs:904464
UUID:
uuid:801f1a3a-8fc5-4fa5-b464-a1c0ac42e99e
Local pid:
pubs:904464
Source identifiers:
904464
Deposit date:
2018-08-10

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