Dataset
Holistic assessment of the microbiome dynamics in the substrates used for commercial champignon (Agaricus bisporus) cultivation
- Alternative title:
- Microbiome dynamics for champignon cultivation
- Documentation:
- Samples were destructively taken from the compost at 5 different stages (G1: phase I; G2: phase II; G3: phase III; G8: flush 1; G10: flush 2) along the composting process and cropping period, and from the peat-based casing material, Top-terra® (Legro, Holland), at five successive stages during the cropping cycle (G4: day 1 after the application of the casing; G5: day 6; G6: day 8; G7: flush 1; G9: flush 2). The V3 and V4 region of the bacterial 16S rRNA gene was amplified by PCR using paired end universal bacterial primers, while the internal transcribed spacer (ITS) was amplified for fungi by employing primers that amplify the ITS2 region, ITS3 and ITS4. Metagenomic data were obtained through sequencing performed on an Illumina MiSeq sequencer using a v3 sequencing kit (2x300 cycles).
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Authors/Creators
Contributors
+ Preston, G
- Institution:
- University of Oxford
- Division:
- MPLS
- Department:
- Plant Sciences
- Role:
- Contributor
- ORCID:
- 0000-0003-3882-4438
+ H2020 programme under the Marie Sklodowska-Curie framwork
More from this funder
- Grant:
- Grant agreement no. 742966
- Programme:
- Marie Sklodowska-Curie Individual Fellowship
- Publisher:
- University of Oxford
- Publication date:
- 2020
- Spatial coverage:
- Spain
- Language:
-
English
- Keywords:
- Subjects:
- Deposit date:
-
2020-06-27
Terms of use
- Copyright date:
- 2020
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