Journal article
Measuring Telomerase Activity in Senescent Human T Cells Upon Genetic Modification
- Abstract:
- Telomerase, a RNA-dependent DNA polymerase that adds telomeric DNA at the 3′ ends of eukaryotic chromosomes, is essential for the lifelong preservation of the proliferative potential of antigen specific T lymphocytes. However, senescent T cells that have low telomerase activity, short telomeres and lack of replicative capacity accumulate in old humans, patients with chronic viral infections and cancer. The mechanisms inhibiting telomerase in these cells are poorly understood. Here I describe a strategy that was successfully applied to identify pathways causing telomerase dysfunction in primary human senescent T lymphocytes. Such strategy couples lentiviral vector-based gene manipulations to functional and signaling readouts directly ex vivo, in humans.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
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(Preview, Accepted manuscript, pdf, 151.5KB, Terms of use)
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- Publisher copy:
- 10.1007/978-1-4939-6548-9_10
Authors
- Publisher:
- Springer New York
- Journal:
- Methods Molecular Biology More from this journal
- Volume:
- 1514
- Pages:
- 119-126
- Publication date:
- 2016-10-28
- Acceptance date:
- 2016-10-28
- DOI:
- EISSN:
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1940-6029
- ISSN:
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1064-3745
- Keywords:
- Pubs id:
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pubs:656002
- UUID:
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uuid:7ffed93c-25b5-4025-9cb2-345a434e978c
- Local pid:
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pubs:656002
- Source identifiers:
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656002
- Deposit date:
-
2016-10-31
Terms of use
- Copyright holder:
- Springer Science+Business Media New York
- Copyright date:
- 2016
- Notes:
- © Springer Science+Business Media New York 2016. This is the accepted manuscript version of the article. The final version is available online from Springer at: [10.1007/978-1-4939-6548-9_10]
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