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Single-molecule identification of Coumarin-120 by time-resolved fluorescence detection: Comparison of one- and two-photon excitation in solution

Abstract:

Using two-photon excitation (TPE) at 700 nm as well as one-photon excitation (OPE) at 350 nm, we applied confocal fluorescence microscopy to detect single Coumarin-120 molecules in the solvents water and triacetin. To study the behavior of Coumarin-120 under different excitation conditions, fluorescence lifetimes, multichannel scaler traces, and autocorrelation curves have been measured simultaneously. A signal-to-background ratio of 1300 was achieved for TPE due to a very low background leve...

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Publication status:
Published

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Publisher copy:
10.1021/jp963729w

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Institution:
University of Oxford
Department:
Oxford, MSD, RDM, Molecular Medicine
Role:
Author
Journal:
JOURNAL OF PHYSICAL CHEMISTRY A
Volume:
101
Issue:
24
Pages:
4313-4321
Publication date:
1997-06-12
DOI:
EISSN:
1520-5215
ISSN:
1089-5639
URN:
uuid:7f8b7cdf-f105-49d2-8004-b4000e82e7cc
Source identifiers:
222220
Local pid:
pubs:222220

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