Journal article
A general method for preparing intact nuclear DNA.
- Abstract:
-
Naked nuclear DNA is easily sheared. Two general methods are described for preparing intact DNA in a stable form that can be pipetted without breaking it. Cells are encapsulated in agarose microbeads and then lysed in a non-ionic detergent (i.e., Triton X-100) and 2 M NaCl or an ionic detergent (e.g., sodium or lithium dodecyl sulphate) in low salt. Most cellular protein and RNA then diffuse out through pores in the beads to leave encapsulated and naked DNA which is nevertheless accessible to...
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- Publication status:
- Published
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Bibliographic Details
- Journal:
- EMBO journal
- Volume:
- 3
- Issue:
- 8
- Pages:
- 1837-1842
- Publication date:
- 1984-08-01
- EISSN:
-
1460-2075
- ISSN:
-
0261-4189
- Source identifiers:
-
23816
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:23816
- UUID:
-
uuid:7f8497e0-eb90-4e18-a895-9161f64a9bcb
- Local pid:
- pubs:23816
- Deposit date:
- 2012-12-19
Terms of use
- Copyright date:
- 1984
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