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A general method for preparing intact nuclear DNA.

Abstract:

Naked nuclear DNA is easily sheared. Two general methods are described for preparing intact DNA in a stable form that can be pipetted without breaking it. Cells are encapsulated in agarose microbeads and then lysed in a non-ionic detergent (i.e., Triton X-100) and 2 M NaCl or an ionic detergent (e.g., sodium or lithium dodecyl sulphate) in low salt. Most cellular protein and RNA then diffuse out through pores in the beads to leave encapsulated and naked DNA which is nevertheless accessible to...

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Publication status:
Published

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Institution:
University of Oxford
Division:
MSD
Department:
Pathology Dunn School
Role:
Author
Journal:
EMBO journal
Volume:
3
Issue:
8
Pages:
1837-1842
Publication date:
1984-08-01
EISSN:
1460-2075
ISSN:
0261-4189
Source identifiers:
23816
Language:
English
Keywords:
Pubs id:
pubs:23816
UUID:
uuid:7f8497e0-eb90-4e18-a895-9161f64a9bcb
Local pid:
pubs:23816
Deposit date:
2012-12-19

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