p53 regulates Cdc2 independently of inhibitory phosphorylation to reinforce radiation-induced G2 arrest in human cells.

Journal article

We have investigated the influence of p53 on radiation-induced G2 cell cycle arrest using human H1299 cells expressing temperature-sensitive p53. Gamma-irradiated cells lacking p53 arrested transiently in G2 with Cdc2 extensively phosphorylated at the inhibitory sites Thr14 and Tyr15, and with both Cdc2 and cyclin B1 restricted to the cytoplasm. Activation of p53 by temperature shift resulted in a more protracted G2 arrest that could not be overridden by checkpoint-abrogating drugs. Surprisin...

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Authors: Winters, Z; Ongkeko, WM; Harris, A; Norbury, C

• Publication status: Published
• Journal: Oncogene
• Volume: 17
• Issue: 6
• Pages: 673-684
• Publication date: 1998-08-01
• DOI: 10.1038/sj.onc.1201991
• EISSN: 1476-5594
• ISSN: 0950-9232
• Language: English
• Keywords: Protein Binding; Gamma Rays; Protein Kinases; Tumor Suppressor Protein p53; G2 Phase; Cell Nucleus; Carcinoma, Non-Small-Cell Lung; Biological Transport; CDC2 Protein Kinase; Lung Neoplasms; Cyclin B; Cyclin-Dependent Kinase Inhibitor p21; Cyclins; Humans; Cell Compartmentation