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Functional and molecular determination of carbonic anhydrase levels in bovine and cultured human chondrocytes.

Abstract:

In this study, bovine articular and human chondrocytes from the C-20/A4 cell line were tested for the functional activity and molecular presence of the enzyme carbonic anhydrase. This enzyme is classically considered to be important in the maintenance of high cellular buffering capacity by catalysing the slow attainment of equilibrium between CO(2) and HCO(3)(-). The first functional assay measured the rate of pH equilibration after administration of a fixed dose of CO(2) solution to cell lys...

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Publication status:
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Institution:
University of Oxford
Division:
MSD
Department:
Physiology Anatomy & Genetics
Role:
Author
Journal:
Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology
Volume:
133
Issue:
3
Pages:
427-435
Publication date:
2002-11-01
DOI:
EISSN:
1879-1107
ISSN:
1096-4959
Source identifiers:
113760
Language:
English
Keywords:
Pubs id:
pubs:113760
UUID:
uuid:7e92dd0e-fbe4-4803-907d-a07072acf3fd
Local pid:
pubs:113760
Deposit date:
2013-11-17

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