SPINE high-throughput crystallization, crystal imaging and recognition techniques: current state, performance analysis, new technologies and future aspects.

Journal article

This paper reviews the developments in high-throughput and nanolitre-scale protein crystallography technologies within the remit of workpackage 4 of the Structural Proteomics In Europe (SPINE) project since the project's inception in October 2002. By surveying the uptake, use and experience of new technologies by SPINE partners across Europe, a picture emerges of highly successful adoption of novel working methods revolutionizing this area of structural biology. Finally, a forward view is taken of how crystallization methodologies may develop in the future.

Authors: Berry, I; Dym, O; Esnouf, R; Harlos, K; Meged, R

• Publication status: Published
• Journal: Acta crystallographica. Section D, Biological crystallography
• Volume: 62
• Issue: Pt 10
• Pages: 1137-1149
• Publication date: 2006-10-01
• DOI: 10.1107/s090744490602943x
• EISSN: 1399-0047
• ISSN: 0907-4449
• Language: English
• Keywords: Robotics; Plastics; Quality Control; Proteins; Nanotechnology; Crystallography; Image Processing, Computer-Assisted; Proteomics