Cisplatin and phenanthriplatin modulate long-noncoding RNA expression in A549 and IMR90 cells revealing regulation of microRNAs, Wnt/β-catenin and TGF-β signaling

Monroe, JD; Moolani, SA; Irihamye, EN; Lett, KE; Hebert, MD; Gibert, Y; Smith, ME

Journal article

Cisplatin and phenanthriplatin modulate long-noncoding RNA expression in A549 and IMR90 cells revealing regulation of microRNAs, Wnt/β-catenin and TGF-β signaling

Abstract:: Lung cancer cells tend to develop resistance to cisplatin (DDP) during continuous chemotherapy, making it crucial to improve DDP sensitivity to enhance therapeutic outcomes. The levels of miR-149-3p in lung tissues and cells, as well as the biological behaviors of lung cancer cells, were analyzed. H446/DDP and A549/DDP cell lines were established to investigate how miR-149-3p affects lung cancer cells’ sensitivity to DDP. Bioinformatics analysis predicted transmembrane serine protease 4 (TMPRSS4) as a downstream target of miR-149-3p, which was subsequently confirmed. Western blot analysis was used to examine proteins related to migration, invasion, apoptosis, and TMPRSS4 expression. Additionally, a subcutaneous graft tumor model in nude mice was created to assess the impact of miR-149-3p on tumor growth. In lung cancer tissues and cells, miR-149-3p expression was reduced, while TMPRSS4 expression was elevated. Overexpression of miR-149-3p inhibited cancer progression, promoted apoptosis, and enhanced the chemosensitivity of lung cancer cells to DDP. Moreover, miR-149-3p negatively regulated TMPRSS4, reducing malignancy-associated characteristics of lung cancer cells and further improving their DDP sensitivity. In vivo, high miR-149-3p expression increased the chemosensitivity of cancer cells. In conclusion, miR-149-3p suppresses the aggressive progression of lung cancer by directly downregulating TMPRSS4 and enhances the responsiveness of lung cancer cells to DDP

Publication status:: Published

Peer review status:: Peer reviewed

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APA Style

Monroe, J. D., Moolani, S. A., Irihamye, E. N., Lett, K. E., Hebert, M. D., Gibert, Y., & Smith, M. E. (2021). Cisplatin and phenanthriplatin modulate long-noncoding RNA expression in A549 and IMR90 cells revealing regulation of microRNAs, Wnt/β-catenin and TGF-β signaling. Scientific Reports, 11(1), 10408–10408.

MLA Style

Monroe, JD, et al. “Cisplatin and Phenanthriplatin Modulate Long-Noncoding RNA Expression in A549 and IMR90 Cells Revealing Regulation of MicroRNAs, Wnt/β-Catenin and TGF-β Signaling.” Scientific Reports, vol. 11, no. 1, 2021, pp. 10408–08.

Chicago Style

Monroe, JD, SA Moolani, EN Irihamye, et al. 2021. “Cisplatin and Phenanthriplatin Modulate Long-Noncoding RNA Expression in A549 and IMR90 Cells Revealing Regulation of MicroRNAs, Wnt/β-Catenin and TGF-β Signaling.” Scientific Reports 11 (1): 10408–.
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