Hypoxia-inducible factor prolyl hydroxylase 2 has a high affinity for ferrous iron and 2-oxoglutarate.

Journal article

Regulation of the hypoxic response in humans is regulated by the post-translational hydroxylation of hypoxia inducible transcription factor; a recombinant form of a human prolyl-4-hydroxylase (PHD2) was characterised and shown to have an unexpectedly high affinity for, and to copurify with endogenous levels of, its Fe(ii) cofactor and 2-oxoglutarate cosubstrate.

Authors: McNeill, L; Flashman, E; Buck, MR; Hewitson, K; Clifton, I

• Publication status: Published
• Journal: Molecular bioSystems
• Volume: 1
• Issue: 4
• Pages: 321-324
• Publication date: 2005-10-01
• DOI: 10.1039/b511249b
• EISSN: 1742-2051
• ISSN: 1742-206X
• Language: English
• Keywords: Mass Spectrometry; Hypoxia-Inducible Factor 1; Models, Molecular; Procollagen-Proline Dioxygenase; Ferrous Compounds; Humans; Chromatography, Liquid; Ketoglutaric Acids; Binding Sites