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Characterization of nuclear structures containing superhelical DNA.

Abstract:

Structures resembling nuclei but depleted of protein may be released by gently lysing cells in solutions containing non-ionic detergents and high concentrations of salt. These nucleoids sediment in gradients containing intercalating agents in a manner characteristic of DNA that is intact, supercoiled and circular. The concentration of salt present during isolation of human nucleoids affects their protein content. When made in I-95 M NaCl they lack histones and most of the proteins characteris...

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Publication status:
Published

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Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
Brazell, IA More by this author
Journal:
Journal of cell science
Volume:
22
Issue:
2
Pages:
303-324
Publication date:
1976-11-05
EISSN:
1477-9137
ISSN:
0021-9533
URN:
uuid:7ce66bf3-c7c5-4e8e-84ff-ee8b2b0af218
Source identifiers:
25108
Local pid:
pubs:25108

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