Characterization of nuclear structures containing superhelical DNA.

Journal article

Structures resembling nuclei but depleted of protein may be released by gently lysing cells in solutions containing non-ionic detergents and high concentrations of salt. These nucleoids sediment in gradients containing intercalating agents in a manner characteristic of DNA that is intact, supercoiled and circular. The concentration of salt present during isolation of human nucleoids affects their protein content. When made in I-95 M NaCl they lack histones and most of the proteins characteris...

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Authors: Cook, P; Brazell, I; Jost, E

• Publication status: Published
• Journal: Journal of cell science
• Volume: 22
• Issue: 2
• Pages: 303-324
• Publication date: 1976-11-01
• EISSN: 1477-9137
• ISSN: 0021-9533
• Language: English
• Keywords: DNA; Pronase; Acridines; Cell Nucleus; RNA; Nucleoproteins; Ribonucleases; Cell Fractionation; Nucleic Acid Conformation; Centrifugation, Density Gradient; Sodium Chloride; Cells, Cultured; Hela Cells; Species Specificity; Gamma Rays; Temperature; Microscopy, Fluorescence