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Journal article

Using Laponite to deliver BMP‐2 for bone tissue engineering – in vitro, chorioallantoic membrane assay and murine subcutaneous model validation

Abstract:
Fracture non-union occurs due to various factors, leading to the development of potentially substantial bone defects. While autograft and allograft are the current gold standards for non-union fractures, challenges related to availability and immune rejection highlight the need for improved treatments. A strategy in bone tissue engineering is to harness growth factors to induce an effect on cells to change their phenotype, behavior and initiate signaling pathways which lead to increased matrix deposition and tissue formation. Bone morphogenetic protein-2 (BMP-2) is a potent osteogenic growth factor however, given its rapid clearance time in vivo, there is a specific therapeutic window for efficacy while avoiding potential deleterious side-effects. It is demonstrated that a Laponite nanoclay coating on a 3D printable and bioresorbable poly(caprolactone) trimethacrylate-based resin enables binding of BMP-2, decreases the rate of release, enabling reduced concentrations to be used while enhancing osteoinduction in both in vitro and in vivo models.
Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1002/admi.202400332

Authors

More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Physiology Anatomy and Genetics
Role:
Author
ORCID:
0000-0002-6272-515X
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Physiology Anatomy and Genetics
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Physiology Anatomy and Genetics
Role:
Author


More from this funder
Funder identifier:
https://ror.org/03x94j517
Grant:
MR/R015651/1
More from this funder
Funder identifier:
https://ror.org/001aqnf71
Grant:
EP/X027163/1
More from this funder
Funder identifier:
https://ror.org/00cwqg982
Grant:
BB/R015651/1


Publisher:
Wiley
Journal:
Advanced Materials Interfaces More from this journal
Volume:
11
Issue:
29
Article number:
2400332
Publication date:
2024-07-29
Acceptance date:
2024-07-16
DOI:
EISSN:
2196-7350


Language:
English
Keywords:
Pubs id:
2022662
Local pid:
pubs:2022662
Deposit date:
2025-07-04
ARK identifier:

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