Journal article
Model-driven experimentation: A new approach to understand mechanisms of tertiary lymphoid tissue formation, function, and therapeutic resolution
- Abstract:
- The molecular and cellular processes driving the formation of secondary lymphoid tissues have been extensively studied using a combination of mouse knockouts, lineage-specific reporter mice, gene expression analysis, immunohistochemistry, and flow cytometry. However, the mechanisms driving the formation and function of tertiary lymphoid tissue (TLT) experimental techniques have proven to be more enigmatic and controversial due to differences between experimental models and human disease pathology. Systems-based approaches including data-driven biological network analysis (gene interaction network, metabolic pathway network, cell-cell signaling, and cascade networks) and mechanistic modeling afford a novel perspective from which to understand TLT formation and identify mechanisms that may lead to the resolution of tissue pathology. In this perspective, we make the case for applying model-driven experimentation using two case studies, which combined simulations with experiments to identify mechanisms driving lymphoid tissue formation and function, and then discuss potential applications of this experimental paradigm to identify novel therapeutic targets for TLT pathology.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 577.2KB, Terms of use)
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- Publisher copy:
- 10.3389/fimmu.2016.00658
Authors
- Publisher:
- Frontiers Media
- Journal:
- Frontiers in Immunology More from this journal
- Volume:
- 7
- Issue:
- APR
- Pages:
- 658
- Publication date:
- 2017-04-04
- Acceptance date:
- 2016-12-16
- DOI:
- EISSN:
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1664-3224
- Pmid:
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28421068
- Language:
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English
- Keywords:
- Pubs id:
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pubs:821758
- UUID:
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uuid:7c8c2c2d-6cb7-4433-828b-b40e6a40bbca
- Local pid:
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pubs:821758
- Source identifiers:
-
821758
- Deposit date:
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2018-05-17
- ARK identifier:
Terms of use
- Copyright holder:
- Coles et al
- Copyright date:
- 2017
- Notes:
- Copyright © 2017 Butler, Cosgrove, Alden, Timmis and Coles. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
- Licence:
- CC Attribution (CC BY)
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