Delaminated layered double hydroxide nanosheets as an efficient vector for DNA delivery

Wang, J; Bao, W; Umar, A; Wang, Q; O'Hare, D; Wan, Y

Journal article

Delaminated layered double hydroxide nanosheets as an efficient vector for DNA delivery

Abstract:: The performance of delaminated Mg–Al-lactate and Mg–Al-acetate layered double hydroxides (LDHs) nanosheets (Mg–Al-lactate-NS, Mg–Al-acetate-NS) as efficient vectors for DNA adsorption and delivery to 293T cells was investigated. Mg–Al-lactate and Mg–Al-acetate LDHs were delaminated into single layers in water and were characterized using XRD, SEM, TEM, and Zeta potential measurements. The salmon sperm DNA adsorption capacity of Mg–Al-lactate-NS and Mg–Al-acetate-NS were evaluated by varying the adsorbent dosage and contacting time, which suggested that Mg–Al-lactate-NS had much higher adsorption capacity (649.6 μg mg–1) than that of Mg–Al-acetate-NS (340.0 μg mg–1. XRD analysis indicated that after DNA adsorption the Mg–Al-lactate-NS-DNA bio-inorganic nanohybrid still stayed in an exfoliated form. Due to the difficulty in separating the Mg–Al-lactate-NS-DNA from solution, electrophoresis analysis was also applied to detect the free DNA in solution after adsorption. Cytotoxicity studies using 293T cells verified that Mg–Al-lactate-NS was less toxic than Mg–Al-acetate-NS as a smaller dose of this LDH was needed to deliver the same amount of salmon DNA to 293T cells. Cellular uptake and confocal imaging studies demonstrated that Mg–Al-lactate-NS was successful in transfection of ssDNA-FITC into 293T cells. However, the FITC-coupled single strand DNA was failed to be internalized into these cells. The excellent DNA adsorption and delivery capacities indicate that delaminated Mg–Al-lactate LDHs nanosheets are a better DNA vector than bulk phase LDH.

Publication status:: Published

Peer review status:: Peer reviewed

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APA Style

Wang, J., Bao, W., Umar, A., Wang, Q., O'Hare, D., & Wan, Y. (2016). Delaminated layered double hydroxide nanosheets as an efficient vector for DNA delivery. Journal of Biomedical Nanotechnology, 12(5), 922–933.

MLA Style

Wang, J., et al. “Delaminated Layered Double Hydroxide Nanosheets as an Efficient Vector for DNA Delivery.” Journal of Biomedical Nanotechnology, vol. 12, no. 5, American Scientific Publishers, 2016, pp. 922–33.

Chicago Style

Wang, J, W Bao, A Umar, Q Wang, D O'Hare, and Y Wan. 2016. “Delaminated Layered Double Hydroxide Nanosheets as an Efficient Vector for DNA Delivery.” Journal of Biomedical Nanotechnology 12 (5): 922–33.
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