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Structural diversity of native major ampullate, minor ampullate, cylindriform, and flagelliform silk proteins in solution

Abstract:

The foundations of silk spinning, the structure, storage, and activation of silk proteins, remain highly debated. By combining solution small-angle neutron and X-ray scattering (SANS and SAXS) alongside circular dichroism (CD), we reveal a shape anisotropy of the four principal native spider silk feedstocks from Nephila edulis. We show that these proteins behave in solution like elongated semiflexible polymers with locally rigid sections. We demonstrated that minor ampullate and cylindriform ...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1021/acs.biomac.0c00819

Authors


Publisher:
American Chemical Society
Journal:
Biomacromolecules More from this journal
Volume:
21
Issue:
8
Pages:
3387–3393
Publication date:
2020-07-08
Acceptance date:
2020-07-08
DOI:
EISSN:
1526-4602
ISSN:
1525-7797
Language:
English
Keywords:
Pubs id:
1117072
Local pid:
pubs:1117072
Deposit date:
2020-07-08

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