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A streamlined implementation of the glutamine synthetase-based protein expression system.

Abstract:

BACKGROUND: The glutamine synthetase-based protein expression system is widely used in industry and academia for producing recombinant proteins but relies on the cloning of transfected cells, necessitating substantial investments in time and handling. We streamlined the production of protein-producing cultures of Chinese hamster ovary cells using this system by co-expressing green fluorescent protein from an internal ribosomal entry site and selecting for high green fluorescent protein-expres...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1186/1472-6750-13-74

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Institution:
University of Oxford
Role:
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Institution:
University of Oxford
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Institution:
University of Oxford
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Institution:
University of Oxford
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Institution:
University of Oxford
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Medical Research Council More from this funder
Biotechnology and Biological Sciences Research Council More from this funder
Publisher:
BioMed Central Ltd. Publisher's website
Journal:
BMC biotechnology Journal website
Volume:
13
Issue:
1
Pages:
74
Publication date:
2013-01-01
DOI:
EISSN:
1472-6750
ISSN:
1472-6750
URN:
uuid:7ad0e202-6a5b-4958-9226-c514e0540349
Source identifiers:
431097
Local pid:
pubs:431097

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