- Abstract:
-
BACKGROUND: The glutamine synthetase-based protein expression system is widely used in industry and academia for producing recombinant proteins but relies on the cloning of transfected cells, necessitating substantial investments in time and handling. We streamlined the production of protein-producing cultures of Chinese hamster ovary cells using this system by co-expressing green fluorescent protein from an internal ribosomal entry site and selecting for high green fluorescent protein-expres...
Expand abstract - Publication status:
- Published
- Peer review status:
- Peer reviewed
- Version:
- Publisher's version
- Files:
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(doc, 47.5KB)
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(bin, 322.0KB)
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(pdf, 652.9KB)
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(pdf, 20.9KB)
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(pdf, 21.3KB)
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- Publisher copy:
- 10.1186/1472-6750-13-74
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- Publisher:
- BioMed Central Ltd. Publisher's website
- Journal:
- BMC biotechnology Journal website
- Volume:
- 13
- Issue:
- 1
- Pages:
- 74
- Publication date:
- 2013-01-01
- DOI:
- EISSN:
-
1472-6750
- ISSN:
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1472-6750
- URN:
-
uuid:7ad0e202-6a5b-4958-9226-c514e0540349
- Source identifiers:
-
431097
- Local pid:
- pubs:431097
- Copyright holder:
- Knox et al.
- Copyright date:
- 2013
- Notes:
- © 2013 Knox et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Journal article
A streamlined implementation of the glutamine synthetase-based protein expression system.
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Medical Research Council
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Biotechnology and Biological Sciences Research Council
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