Journal article

Aberrations in 4Pi microscopy.

Abstract:: The combination of two opposing objective lenses in 4Pi fluorescence microscopy significantly improves the axial resolution and increases the collection efficiency. Combining 4Pi microscopy with other super-resolution techniques has resulted in the highest three-dimensional (3D) resolution in fluorescence microscopy to date. It has previously been shown that the performance of 4Pi microscopy is significantly affected by aberrations. However, a comprehensive description of 4Pi microscope aberrations has been missing. In this paper, we introduce an approach to describe aberrations in a 4Pi cavity through a new functional representation. We discuss the focusing properties of 4Pi systems affected by aberrations and discuss the implications for adaptive optics schemes for 4Pi microscopes based on this new insight.

Publication status:: Published

Peer review status:: Peer reviewed

Actions

Email

Email this record

Send the bibliographic details of this record to your email address.

Your Email
Please enter the email address that the record information will be sent to.

-
Your message (optional)
Please add any additional information to be included within the email.
Share
Cite

Cite this record

APA Style

Hao, X., Antonello, J., Allgeyer, E., Bewersdorf, J., & Booth, M. (2017). Aberrations in 4Pi microscopy. Optics Express, 25(13), 14049–14058.

MLA Style

Hao, X, et al. “Aberrations in 4Pi Microscopy.” Optics Express, vol. 25, no. 13, 2017, pp. 14049–58.

Chicago Style

Hao, X, J Antonello, E Allgeyer, J Bewersdorf, and M Booth. 2017. “Aberrations in 4Pi Microscopy.” Optics Express 25 (13): 14049–58.
Print

Access Document

Files:: Antonello et al, Aberrations in 4Pi microscopy.pdf

(Preview, Version of record, pdf, 2.9MB, Terms of use)

Publisher copy:: 10.1364/oe.25.014049

Authors

+ Hao, X More by this author

Role:: Author

+ Antonello, J More by this author

Institution:: University of Oxford
Division:: MSD
Department:: Physiology Anatomy & Genetics
Role:: Author

+ Allgeyer, E More by this author

Role:: Author

+ Bewersdorf, J More by this author

Role:: Author

+ Booth, M More by this author

Institution:: University of Oxford
Division:: MPLS
Department:: Engineering Science
Role:: Author

+ Wellcome Trust More from this funder

Grant:: 203285/C/16/Z

+ European Research Council More from this funder

Grant:: 695140

+ National Institutes of Health More from this funder

Grant:: P30 DK45735

+ G. Harold and Leila Y. Mathers Foundation More from this funder

Publisher:: Optical Society
Journal:: Optics Express More from this journal
Volume:: 25
Issue:: 13
Pages:: 14049-14058
Publication date:: 2017-06-01
Acceptance date:: 2017-05-24
DOI:: 10.1364/oe.25.014049
EISSN:: 1094-4087
Pmid:: 28788990

Language:: English
Keywords:: Journal Article
Pubs id:: pubs:704660
UUID:: uuid:79c652f7-f1a3-4807-82cd-93ad43affcd6
Local pid:: pubs:704660
Source identifiers:: 704660
Deposit date:: 2017-08-17
ARK identifier:: ark:/29072/ora_79c652f7f1a3480782cd93ad43affcd6

Terms of use

Copyright holder:: Optical Society of America
Copyright date:: 2017
Notes:: © 2017 Optical Society of America

Licence:: Terms and Conditions of Use for Oxford University Research Archive

Views and Downloads

About views and downloads

If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP