Journal article
Super-resolution microscopy demystified
- Abstract:
- Super-resolution microscopy (SRM) bypasses the diffraction limit, a physical barrier that restricts the optical resolution to roughly 250 nm and was previously thought to be impenetrable. SRM techniques allow the visualization of subcellular organization with unprecedented detail, but also confront biologists with the challenge of selecting the best-suited approach for their particular research question. Here, we provide guidance on how to use SRM techniques advantageously for investigating cellular structures and dynamics to promote new discoveries.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
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(Preview, Accepted manuscript, pdf, 7.7MB, Terms of use)
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- Publisher copy:
- 10.1038/s41556-018-0251-8
Authors
- Publisher:
- Nature Research
- Journal:
- Nature Cell Biology More from this journal
- Volume:
- 21
- Issue:
- 1
- Pages:
- 72-84
- Publication date:
- 2019-01-02
- Acceptance date:
- 2018-11-12
- DOI:
- EISSN:
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1476-4679
- ISSN:
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1465-7392
- Pmid:
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30602772
- Language:
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English
- Keywords:
- Pubs id:
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pubs:959237
- UUID:
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uuid:799d6925-11e1-4e45-a6d9-9e5aa5b5f9cf
- Local pid:
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pubs:959237
- Source identifiers:
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959237
- Deposit date:
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2019-07-01
- ARK identifier:
Terms of use
- Copyright holder:
- Springer Nature Limited
- Copyright date:
- 2019
- Notes:
- © Springer Nature Limited 2019. This is the accepted manuscript version of the article. The final version is available online from Nature Research at: https://doi.org/10.1038/s41556-018-0251-8.
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