Genetic aberrations in glioblastoma multiforme: translocation of chromosome 10 in an O-2A-like cell line.

Journal article

We have examined the genetic aberrations in two near-diploid glioblastoma multiforme cell lines that appear to have arisen from different glial lineages. One cell line, Hu-O-2A/Gb1, expresses antigens and metabolic profiles characteristic of the oligodendrocyte-type-2 astrocyte (0-2A) lineage of the rat central nervous system. This line generates, in vitro, cells with characteristics of 0-2A progenitor cells, oligodendrocytes and astrocytes. The second cell line, IN1434, is derived from an astrocyte or a precursor cell restricted to astrocytic differentiation. In Hu-O-2A/Gb1 the sole homologue of chromosome 10 is disrupted at band 10p11-12.1 by translocation with chromosomes X and 15. The translocation breakpoint is localized between genetic markers D10S2103 and [D10S637, D10S1962, D10S355]. Other aberrations include a 5;14 translocation, deletion of the long and short arms of chromosome 16 and loss of one copy of the CDKN2 gene. IN1434 cells share some cytogenetic abnormalities with Hu-O-2A/Gb1 cells, despite their apparent derivation from a different biological origin, but also have translocations involving the long and short arms of chromosome 1 and the long arm of chromosome 7, and deletion of chromosome 13 at bands 13q12-21.

Authors: Mao, X; Jones, T; Tomlinson, I; Rowan, A; Fedorova, L

• Publication status: Published
• Journal: British journal of cancer
• Volume: 79
• Issue: 5-6
• Pages: 724-731
• Publication date: 1999-02-01
• DOI: 10.1038/sj.bjc.6690116
• EISSN: 1532-1827
• ISSN: 0007-0920
• Language: English
• Keywords: Cell Line; Male; Rats; Brain Neoplasms; Translocation, Genetic; Chromosome Aberrations; Tumor Cells, Cultured; Middle Aged; Chromosomes, Human, Pair 10; Astrocytes; Glioblastoma; Chromosome Mapping; Oligodendroglia; Humans; Karyotyping; Animals; Microsatellite Repeats