Journal article
TCF-ALP: a fluorescent probe for the selective detection of <i>Staphylococcus</i> bacteria and application in “smart” wound dressings
- Abstract:
- Alkaline phosphatase (ALP) is an important enzyme-based biomarker present in several bacterial species; however, it is currently undervalued as a strategy to detect pathogenic bacteria. Here, we explore our ALP-responsive colorimetric and fluorescent probe (TCF-ALP) for such applications. TCF-ALP displayed a colorimetric and fluorescence response towards Staphylococcus aureus (S. aureus), with a limit of detection of 3.7 × 106 CFU mL−1 after 24 h incubation. To our surprise, TCF-ALP proved selective towards Staphylococcus bacteria when compared with Enterococcus faecalis (E. faecalis), and Gram-negative P. aeruginosa and E. coli. Selectivity was also seen in clinically relevant S. aureus biofilms. Owing to the high prevalence and surface location of S. aureus in chronic wounds, TCF-ALP was subsequently encapsulated in polyvinyl alcohol (PVA)-based hydrogels as a proof-of-concept “smart” wound dressing. TCF-ALP hydrogels were capable of detecting S. aureus in planktonic and biofilm assays, and displayed a clear colour change from yellow to purple after 24 h incubation using ex vivo porcine skin models. Overall, TCF-ALP is a simple tool that requires no prior knowledge, training, or specialist equipment, and has the potential to overcome issues related to invasive swabbing and tissue biopsy methods. Thus, TCF-ALP could be used as a tool to monitor the early development of infection in a wound and allow for the rapid provision of appropriate treatment for Staphylococcal bacterial infections
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 1.4MB, Terms of use)
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- Publisher copy:
- 10.1039/d0bm01918f
Authors
+ Medical Research Council
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- Funder identifier:
- https://ror.org/03x94j517
- Grant:
- MR/N0137941/1
+ Henan Normal University
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- Funder identifier:
- 10.13039/100009555
- Grant:
- 2020ZD01
+ Fundamental Research Funds for the Central Universities
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- Funder identifier:
- 10.13039/501100012226
- Grant:
- 222201717003
+ Science and Technology Commission of Shanghai Municipality
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- Funder identifier:
- 10.13039/501100003399
- Grant:
- 17520750100
+ National Natural Science Foundation of China
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- Funder identifier:
- 10.13039/501100001809
- Grant:
- 21788102
- Publisher:
- Royal Society of Chemistry
- Journal:
- Biomaterials Science More from this journal
- Volume:
- 9
- Issue:
- 12
- Pages:
- 4433-4439
- Publication date:
- 2021-06-15
- DOI:
- EISSN:
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2047-4849
- ISSN:
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2047-4830
- Language:
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English
- Keywords:
- Pubs id:
-
1301982
- Local pid:
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pubs:1301982
- Source identifiers:
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W3162657751
- Deposit date:
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2026-04-29
- ARK identifier:
This ORA record was generated from metadata provided by an external service. It has not been edited by the ORA Team.
Terms of use
- Copyright date:
- 2021
- Licence:
- CC Attribution (CC BY)
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