Ex vivo T-cell depletion with the monoclonal antibody Campath-1 plus human complement effectively prevents acute graft-versus-host disease in allogeneic bone marrow transplantation.

Journal article

We have developed a rapid and simple procedure for the elimination of mature T-cells from the donor marrow using a single incubation with the monoclonal antibody Campath-1 and donor complement. This resulted in a reduction of T-cell contamination to a mean of 1%. This regimen reduced the incidence of acute graft-versus-host disease significantly in 21 consecutive bone marrow grafts in 18 patients with leukaemia and non-Hodgkin's lymphoma. Purging was responsible for an increased incidence of graft rejection in HLA-identical transplants (13%).

Authors: Heit, W; Bunjes, D; Wiesneth, M; Schmeiser, T; Arnold, R

• Publication status: Published
• Journal: British journal of haematology
• Volume: 64
• Issue: 3
• Pages: 479-486
• Publication date: 1986-11-01
• DOI: 10.1111/j.1365-2141.1986.tb02203.x
• EISSN: 1365-2141
• ISSN: 0007-1048
• Language: English
• Keywords: T-Lymphocytes; Adolescent; Adult; Cell Separation; Complement System Proteins; Bone Marrow Transplantation; Graft vs Host Disease; Antibodies, Monoclonal; Male; Bone Marrow Cells; Humans; Bone Marrow; Female