Electron fourier ptychography for phase reconstruction

Journal article

Exit wavefunction reconstruction is important in transmission electron microscopy for structural studies. We describe electron Fourier ptychography and its application to phase reconstruction of both radiation-resistant and beam-sensitive materials. We demonstrate that the phase of the exit wave can be reconstructed to high resolution using a modified iterative phase retrieval algorithm from data collected in an alternative optical geometry. This method achieves a spatial resolution of 0.63 nm at a fluence of 4.5 × 102 e−/nm2, as validated on Cry11Aa protein crystals under cryogenic conditions. Notably, this method requires no instrumental modifications, is straightforward to implement, and can be seamlessly integrated with existing data collection software, providing a broadly accessible alternative approach for structural studies.

Authors: Zhao, J; Huang, C; Mostaed, A; Moshtaghpour, A; Parkhurst, JM

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: Nature Research
• Journal: Scientific Reports
• Volume: 15
• Issue: 1
• Article number: 37955
• Publication date: 2025-10-30
• Acceptance date: 2025-09-22
• DOI: 10.1038/s41598-025-21638-7
• EISSN: 2045-2322
• ISSN: 2045-2322
• Language: English
• Keywords: Low fluence; Cryogenic condition; Electron Fourier ptychography; Phase reconstruction