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Generation of a synthetic GlcNAcylated nucleosome reveals regulation of stability by H2A-Thr101 GlcNAcylation

Abstract:

O-GlcNAcylation is a newly discovered histone modification implicated in transcriptional regulation, but no structural information on the physical effect of GlcNAcylation on chromatin exists. Here, we generate synthetic, pure GlcNAcylated histones and nucleosomes and reveal that GlcNAcylation can modulate structure through direct destabilization of H2A/H2B dimers in the nucleosome, thus promoting an 'open' chromatin state. The results suggest that a plausible molecular basis for one role of h...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1038/ncomms8978

Authors


Lercher, L More by this author
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Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Physical and Theoretical Chem
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Publisher:
Nature Publishing Group Publisher's website
Journal:
Nature communications Journal website
Volume:
6
Pages:
7978
Publication date:
2015-08-25
Acceptance date:
2015-07-02
DOI:
EISSN:
2041-1723
ISSN:
2041-1723
URN:
uuid:781cd45f-029c-45f0-bcf7-3ba8e9f99e99
Source identifiers:
541723
Local pid:
pubs:541723

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