Understanding non-coding DNA regions in yeast.

Journal article

Non-coding transcripts play an important role in gene expression regulation in all species, including budding and fission yeast. Such regulatory transcripts include intergenic ncRNA (non-coding RNA), 5' and 3' UTRs, introns and antisense transcripts. In the present review, we discuss advantages and limitations of recently developed sequencing techniques, such as ESTs, DNA microarrays, RNA-Seq (RNA sequencing), DRS (direct RNA sequencing) and TIF-Seq (transcript isoform sequencing). We provide an overview of methods applied in yeast and how each of them has contributed to our knowledge of gene expression regulation and transcription.

Authors: Schlackow, M; Gullerova, M

• Publication status: Published
• Journal: Biochemical Society transactions
• Volume: 41
• Issue: 6
• Pages: 1654-1659
• Publication date: 2013-12-01
• DOI: 10.1042/bst20130144
• EISSN: 1470-8752
• ISSN: 0300-5127
• Language: English
• Keywords: Transcription, Genetic; Gene Expression Regulation, Fungal; Saccharomyces cerevisiae; RNA, Untranslated; DNA, Fungal