2-D protein maps of rat gastrocnemius and soleus muscles: a tool for muscle plasticity assessment.

Gelfi, C; Viganò, A; De Palma, S; Ripamonti, M; Begum, S; Cerretelli, P; Wait, R

Journal article

2-D protein maps of rat gastrocnemius and soleus muscles: a tool for muscle plasticity assessment.

Abstract:: Functional characterization of muscle fibers relies on ATPase activity and on differential measurements of metabolic proteins, including mitochondrial and glycolytic enzymes, glucose, lactate and lactic acid transporters, calcium cycling proteins and components of the contractile machinery. The recent introduction of microarray technology has enabled detailed gene expression studies under different physiological and pathological conditions, thus generating novel hypotheses on muscle function. However, microarray approaches are limited by the incomplete genome coverage of currently available chips, and by poor correlation between mRNA concentration and protein expression level. We have used 2-DE and MS to build a reference map of proteins from rat mixed gastrocnemius and soleus muscle, and to assess qualitative and quantitative differences in protein distribution between these two functionally dissimilar muscles. More than 800 spots on each gel were detected by silver staining, of which 167 were excised, digested in-gel with trypsin and analyzed by ESI-MS/MS. One hundred and twenty eight distinct gene products were identified, including metabolic, transport and contractile proteins. Forty one spots displayed differences in relative expression level between mixed gastrocnemius and soleus samples. These data not only enable differentiation of functionally distinct slow-twitch and fast-twitch fiber types, but also provide tools for investigating muscle plasticity in response to physiological and environmental conditions such as aging or hypoxia.

Publication status:: Published

Actions

Email

Email this record

Send the bibliographic details of this record to your email address.

Your Email
Please enter the email address that the record information will be sent to.

-
Your message (optional)
Please add any additional information to be included within the email.
Cite

Cite this record

APA Style

Gelfi, C., Viganò, A., De Palma, S., Ripamonti, M., Begum, S., Cerretelli, P., & Wait, R. (2006). 2-D protein maps of rat gastrocnemius and soleus muscles: a tool for muscle plasticity assessment. Proteomics, 6(1), 321–340.

MLA Style

Gelfi, C., et al. “2-D Protein Maps of Rat Gastrocnemius and Soleus Muscles: a Tool for Muscle Plasticity Assessment.” Proteomics, vol. 6, no. 1, 2006, pp. 321–40.

Chicago Style

Gelfi, C, A Viganò, S De Palma, M Ripamonti, S Begum, P Cerretelli, and R Wait. 2006. “2-D Protein Maps of Rat Gastrocnemius and Soleus Muscles: a Tool for Muscle Plasticity Assessment.” Proteomics 6 (1): 321–40.
Share
Print