Assay and functional properties of Rabkinesin-6/Rab6-KIFL/MKlp2 in cytokinesis.

Journal article

Here we describe methods for the characterization of the kinesin-9 family motor protein Rabkinesin-6/Rab6-KIFL/MKlp2 in cytokinesis. Here we outline biochemical assays for studying the interaction of Rabkinesin-6/Rab6-KIFL/MKlp2 with microtubules, and its regulation and interaction with the mitotic polo-like kinase 1 using recombinant proteins expressed in and purified from insect cells. Protocols for the in vivo functional analysis of Rabkinesin-6/Rab6-KIFL/MKlp2 using depletion with small interfering RNA duplexes are described.

Authors: Neef, R; Grüneberg, U; Barr, F

• Publication status: Published
• Journal: Methods in enzymology
• Volume: 403
• Pages: 618-628
• Publication date: 2005-01-01
• DOI: 10.1016/s0076-6879(05)03054-5
• EISSN: 1557-7988
• ISSN: 0076-6879
• Language: English
• Keywords: Humans; Animals; RNA Interference; Cell Cycle; Blotting, Western; Spodoptera; Microtubules; Ligands; Phosphorylation; Recombinant Proteins; Kinesin