Journal article : Review
Structural mass spectrometry techniques for characterisation of plant and algal proteins
- Abstract:
- Structural biology can offer valuable insights into the mechanisms and functions of key proteins within plant molecular and cellular systems. However, plant proteins present several specific challenges for structural analysis, including difficulties in expression and purification, significant intrinsic disorder, and extensive post-translational modification. Structural mass spectrometry (MS) offers a complementary set of tools that can help overcome these obstacles and provide detailed structural and mechanistic information. In this review, we outline the principles and practical applications of the main structural mass spectrometry techniques, namely crosslinking, covalent labelling, hydrogen-deuterium exchange, and both intact (denaturing) and native MS. We also discuss recent case studies where structural MS has offered insight into the architecture, dynamics and interactions of proteins central to plant molecular and cell biology.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 1.1MB, Terms of use)
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- Publisher copy:
- 10.1111/tpj.70780
Authors
+ Royal Society
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- Funder identifier:
- https://ror.org/03wnrjx87
- Grant:
- URF\R1\211567
+ European Commission
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- Funder identifier:
- https://ror.org/00k4n6c32
- Grant:
- 101205973
+ UK Research and Innovation
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- Funder identifier:
- https://ror.org/001aqnf71
- Grant:
- EP/Y036158/1
- Publisher:
- Wiley
- Journal:
- Plant Journal More from this journal
- Volume:
- 125
- Issue:
- 6
- Article number:
- e70780
- Place of publication:
- England
- Publication date:
- 2026-03-14
- Acceptance date:
- 2026-02-23
- DOI:
- EISSN:
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1365-313X
- ISSN:
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0960-7412
- Pmid:
-
41830284
- Language:
-
English
- Keywords:
- Subtype:
-
Review
- Pubs id:
-
2390653
- Local pid:
-
pubs:2390653
- Source identifiers:
-
W7135392483
- Deposit date:
-
2026-03-31
- ARK identifier:
Terms of use
- Copyright holder:
- Durant et al.
- Copyright date:
- 2026
- Rights statement:
- ©2026 The Author(s). The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
- Licence:
- CC Attribution (CC BY)
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