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Reverse and multiple stable isotope probing to study bacterial metabolism and interactions at the single cell level

Abstract:

The interactions between microorganisms driven by substrate metabolism and energy flow are important to shape diversity, abundance, and structure of a microbial community. Single cell technologies are useful tools for dissecting the functions of individual members and their interactions in microbial communities. Here, we developed a novel Raman stable isotope probing (Raman-SIP), which uses Raman micro-spectroscopy coupled with reverse and D2O co-labelling to study metabolic interactions in a...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1021/acs.analchem.6b01602

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Department:
Oxford, MPLS, Engineering Science
Role:
Author
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Department:
Oxford, MPLS, Engineering Science
Role:
Author
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Publisher:
American Chemical Society Publisher's website
Journal:
Analytical Chemistry Journal website
Volume:
88
Issue:
19
Pages:
9443–9450
Publication date:
2016-09-05
Acceptance date:
2016-09-02
DOI:
EISSN:
1520-6882)
ISSN:
0003-2700
Pubs id:
pubs:641800
URN:
uri:76590d8d-83f0-4b5e-8287-e7c3d043dcea
UUID:
uuid:76590d8d-83f0-4b5e-8287-e7c3d043dcea
Local pid:
pubs:641800

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