Journal article
Sister chromatid cohesion establishment during DNA replication termination
- Abstract:
- Newly copied sister chromatids are tethered together by the cohesin complex, but how sister chromatid cohesion is coordinated with DNA replication is poorly understood. Prevailing models suggest cohesin complexes, bound to DNA before replication, remain behind the advancing replication fork to keep sister chromatids together. By visualizing single replication forks colliding with pre-loaded cohesin complexes, we find that the replisome instead pushes cohesin to where a converging replisome is met. While the converging replisomes are removed during DNA replication termination, cohesin remains on nascent DNA and provides cohesion. Additionally, we show that CMG disassembly during replication termination is vital for proper cohesion in budding yeast. Together, our results support a new model where sister chromatid cohesion is established during DNA replication termination.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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Access Document
- Files:
-
-
(Preview, Accepted manuscript, pdf, 27.1MB, Terms of use)
-
- Publisher copy:
- 10.1126/science.adf0224
Authors
- Publisher:
- American Association for the Advancement of Science
- Journal:
- Science More from this journal
- Volume:
- 384
- Issue:
- 6691
- Pages:
- 119-124
- Publication date:
- 2024-03-14
- Acceptance date:
- 2024-02-27
- DOI:
- EISSN:
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1095-9203
- ISSN:
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0036-8075
- Language:
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English
- Pubs id:
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1806373
- Local pid:
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pubs:1806373
- Deposit date:
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2024-03-15
Terms of use
- Copyright holder:
- Cameron et al.
- Copyright date:
- 2024
- Rights statement:
- © 2024 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
- Notes:
-
This is the accepted manuscript version of the article. The final version is available online from American Association for the Advancement of Science at https://dx.doi.org/10.1126/science.adf0224
This research was funded in whole or in part by the Wellcome Trust (CC2133, 223235/Z/21/Z, 226494/Z/22/Z), a cOAlition S organization. The author will make the Author Accepted Manuscript (AAM) version available under a CC BY public copyright license.
- Licence:
- CC Attribution (CC BY)
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