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Nuclear/Cytoplasmic fractionation to study Hippo effectors.

Abstract:: The translocation or shuttling of Hippo proteins between the nucleus and cytoplasm is a rapid event following cytoskeletal or mechanical cues as well as stimulation with extracellular growth factors. Here we describe an experimental procedure for a simple and fast separation of nuclear and cytoplasmic fractions which maintains protein integrity and integrity of protein-protein complexes, indicating that it should be applicable to many experimental questions.

Publication status:: Published

Peer review status:: Peer reviewed

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APA Style

Chatzifrangkeskou, M., & O'Neill, E. (2018). Nuclear/Cytoplasmic fractionation to study Hippo effectors. In Methods in Molecular Biology (pp. 115–119). Springer Verlag.

MLA Style

Chatzifrangkeskou, M., and E. O'Neill. “Nuclear/Cytoplasmic Fractionation to Study Hippo Effectors.” Methods in Molecular Biology, Springer Verlag, 2018, pp. 115–19.

Chicago Style

Chatzifrangkeskou, M, and E O'Neill. 2018. “Nuclear/Cytoplasmic Fractionation to Study Hippo Effectors.” In Methods in Molecular Biology, 115–19. Methods in Molecular Biology. Springer Verlag.
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Publisher copy:: 10.1007/978-1-4939-8910-2_10

Authors

+ Chatzifrangkeskou, M More by this author

Institution:: University of Oxford
Division:: Medical Sciences Division
Department:: Oncology
Sub department:: CRUK/MRC Ox Inst Radiation Oncology
Department:: Oxford, MSD, Oncology, CRUK/MRC Ox Inst Radiation Oncology
Role:: Author

+ O'Neill, E More by this author

Institution:: University of Oxford
Division:: Medical Sciences Division
Department:: Oncology
Sub department:: CRUK/MRC Ox Inst Radiation Oncology
Role:: Author

Publisher:: Springer Verlag
Host title:: Methods in Molecular Biology
Pages:: 115-119
Series:: Methods in Molecular Biology
Publication date:: 2018-12-19
DOI:: 10.1007/978-1-4939-8910-2_10
EISSN:: 1940-6029
ISSN:: 1064-3745
Pmid:: 30565130
ISBN:: 9781493989096

Language:: English
Keywords:: hypotonic buffer

cytoplasmic compartment

nuclear compartment

subcellular fractionation

Hippo pathway
Pubs id:: pubs:955193
UUID:: uuid:75557797-1cdd-4b15-8472-99eeb57a768a
Local pid:: pubs:955193
Source identifiers:: 955193
Deposit date:: 2019-06-12

Terms of use

Copyright holder:: Springer
Copyright date:: 2018
Notes:: © Springer Science+Business Media, LLC, part of Springer Nature 2019

Licence:: Terms and Conditions of Use for Oxford University Research Archive

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