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Journal article

Conservation of epigenetic regulation by the MLL3/4 tumour suppressor in planarian pluripotent stem cells

Abstract:
Currently, little is known about the evolution of epigenetic regulation in animal stem cells. Here we demonstrate, using the planarian stem cell system to investigate the role of the COMPASS family of MLL3/4 histone methyltransferases that their function as tumor suppressors in mammalian stem cells is conserved over a long evolutionary distance. To investigate the potential conservation of a genome-wide epigenetic regulatory program in animal stem cells, we assess the effects of Mll3/4 loss of function by performing RNA-seq and ChIP-seq on the G2/M planarian stem cell population, part of which contributes to the formation of outgrowths. We find many oncogenes and tumor suppressors among the affected genes that are likely candidates for mediating MLL3/4 tumor suppression function. Our work demonstrates conservation of an important epigenetic regulatory program in animals and highlights the utility of the planarian model system for studying epigenetic regulation.
Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1038/s41467-018-06092-6

Authors


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Role:
Author
ORCID:
0000-0001-7568-3596
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Institution:
University of Oxford
Division:
MPLS Division
Department:
Zoology
Role:
Author
ORCID:
0000-0002-7489-5164
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
NDM
Sub department:
Target Discovery Institute
Role:
Author


Publisher:
Nature Publishing Group
Journal:
Nature Communications More from this journal
Volume:
9
Issue:
1
Pages:
3633
Publication date:
2018-09-07
Acceptance date:
2018-08-13
DOI:
EISSN:
2041-1723
Pmid:
30194301


Language:
English
Keywords:
Pubs id:
pubs:919112
UUID:
uuid:74efc357-5000-4927-9f26-dcdc41f572dc
Local pid:
pubs:919112
Source identifiers:
919112
Deposit date:
2018-09-17

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