Journal article
Raman-Deuterium Isotope Probing for in-situ identification of antimicrobial resistant bacteria in Thames River
- Abstract:
- The emergence and widespread distribution of antimicrobial resistant (AMR) bacteria has led to an increasing concern with respect to potential environmental and public health risks. Culture-independent and rapid identification of AMR bacteria in-situ in complex environments is important in understanding the role of non-culturable bacteria and in revealing potential pathogens with resistance. In this study, a culture-independent and non-destructive phenotyping approach, so called Raman Deuterium Stable Isotope Probing (Raman-DIP), was developed to identify AMR bacteria in the River Thames. It is demonstrated that Raman-DIP was able to accurately identify resistant and susceptible bacteria within 24 hours. The work shows that, in the River Thames, the majority of the bacteria (76±2%) were metabolically active, whilst AMR bacteria to carbenicillin, kanamycin and both two antibiotics were 35±5%, 28±3%, 25±1% of the total bacteria respectively. Raman activated cell ejection (RACE) was applied to isolate single AMR bacterial cells for the first time, linking AMR phenotype and genotype (DNA sequence). The sequence of the isolates indicates that they were potential human pathogens Aeromonas sp., Stenotrophomonas sp. and an unculturable bacterium. This work demonstrates Raman-DIP and RACE are effective culture-independent approach for rapid identification of AMR bacteria at the single cell level in their natural conditions.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 2.5MB, Terms of use)
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- Publisher copy:
- 10.1038/s41598-017-16898-x
Authors
- Publisher:
- Nature Publishing Group
- Journal:
- Scientific Reports More from this journal
- Publication date:
- 2017-11-30
- Acceptance date:
- 2017-11-10
- DOI:
- EISSN:
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2045-2322
- ISSN:
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2045-2322
- Keywords:
- Pubs id:
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pubs:797223
- UUID:
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uuid:74c1e158-a07b-4d09-b9d7-de0933dab7ff
- Local pid:
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pubs:797223
- Source identifiers:
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797223
- Deposit date:
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2017-11-25
Terms of use
- Copyright holder:
- Huang et al
- Copyright date:
- 2017
- Notes:
- © The Author(s) 2017. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
- Licence:
- CC Attribution (CC BY)
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