Structural basis for amino acid transport by the CAT family of SLC7 transporters

Journal article

Abstract:: Amino acids play essential roles in cell biology as regulators of metabolic pathways. Arginine in particular is a major signalling molecule inside the cell, being a precursor for both l-ornithine and nitric oxide (NO) synthesis and a key regulator of the mTORC1 pathway. In mammals, cellular arginine availability is determined by members of the solute carrier (SLC) 7 family of cationic amino acid transporters. Whereas CAT-1 functions to supply cationic amino acids for cellular metabolism, CAT-2A and -2B are required for macrophage activation and play important roles in regulating inflammation. Here, we present the crystal structure of a close homologue of the mammalian CAT transporters that reveals how these proteins specifically recognise arginine. Our structural and functional data provide a model for cationic amino acid transport in mammalian cells and reveals mechanistic insights into proton-coupled, sodium-independent amino acid transport in the wider APC superfamily.

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Language:: English
Keywords:: Research Support, Non-U.S. Gov't

Ornithine

research-article

Amino Acid Transport Systems, Basic

Arginine

Journal Article

Animals

Nitric Oxide

Crystallization

Crystallography, X-Ray
Pubs id:: pubs:824950
UUID:: uuid:746c26ce-b16d-4136-b96f-95611dde57df
Local pid:: pubs:824950
Source identifiers:: 824950
Deposit date:: 2018-08-11
ARK identifier:: ark:/29072/ora_746c26ceb16d4136b96f95611dde57df

Copyright holder:: Jungnickel et al
Notes:: © The Author(s) 2018. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder

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