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Biochemical identification of nonmethylated DNA by BioCAP-Seq

Abstract:

CpG islands are regions of vertebrate genomes that often function as gene regulatory elements and are associated with most gene promoters. CpG island elements usually contain nonmethylated CpG dinucleotides, while the remainder of the genome is pervasively methylated. We developed a biochemical approach called biotinylated CxxC affinity purification (BioCAP) to unbiasedly isolate regions of the genome that contain nonmethylated CpG dinucleotides. The resulting highly pure nonmethylated DNA is...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Accepted Manuscript

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Publisher copy:
10.1007/978-1-4939-7768-0_2

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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Biochemistry
Role:
Author
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Biochemistry
Role:
Author
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Biochemistry
Oxford college:
Exeter College; Keble College
Role:
Author
ORCID:
0000-0002-8726-7888
Publisher:
Springer Publisher's website
Volume:
1766
Pages:
15-29
Series:
Methods in Molecular Biology
Host title:
CpG Islands
Publication date:
2018-04-01
Acceptance date:
2018-01-01
DOI:
EISBN:
978-1-4939-7768-0
EISSN:
1064-3745
ISSN:
1940-6029
Pubs id:
pubs:833987
URN:
uri:7452ac9c-d3c8-45d4-b36d-86a1df083401
UUID:
uuid:7452ac9c-d3c8-45d4-b36d-86a1df083401
Local pid:
pubs:833987
ISBN:
978-1-4939-7767-3

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