Book section
Biochemical identification of nonmethylated DNA by BioCAP-Seq
- Abstract:
- CpG islands are regions of vertebrate genomes that often function as gene regulatory elements and are associated with most gene promoters. CpG island elements usually contain nonmethylated CpG dinucleotides, while the remainder of the genome is pervasively methylated. We developed a biochemical approach called biotinylated CxxC affinity purification (BioCAP) to unbiasedly isolate regions of the genome that contain nonmethylated CpG dinucleotides. The resulting highly pure nonmethylated DNA is easily analyzed by quantitative PCR to interrogate specific loci or via massively parallel sequencing to yield genome-wide profiles.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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Access Document
- Files:
-
-
(Preview, Accepted manuscript, pdf, 469.5KB, Terms of use)
-
- Publisher copy:
- 10.1007/978-1-4939-7768-0_2
Authors
- Publisher:
- Springer
- Host title:
- Methods in molecular biology
- Volume:
- 1766
- Pages:
- 15-29
- Series:
- Methods in Molecular Biology
- Publication date:
- 2018-04-01
- Acceptance date:
- 2018-01-01
- DOI:
- EISSN:
-
1064-3745
- ISSN:
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1940-6029
- Pmid:
-
29605845
- ISBN:
- 9781493977673
- Language:
-
English
- Keywords:
- Pubs id:
-
pubs:833987
- UUID:
-
uuid:7452ac9c-d3c8-45d4-b36d-86a1df083401
- Local pid:
-
pubs:833987
- Source identifiers:
-
833987
- Deposit date:
-
2018-08-23
Terms of use
- Copyright holder:
- Springer
- Copyright date:
- 2018
- Notes:
- © Springer Science+Business Media, LLC, part of Springer Nature 2018. This is the accepted manuscript version of the article. The final version is available online from Springer at: https://doi.org/10.1007/978-1-4939-7768-0_2
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